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KMID : 0921620080380010029
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Journal of Bacteriology and Virology
2008 Volume.38 No. 1 p.29 ~ p.37
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Intracellular Localization of the Porcine Reproductive and Respiratory Syndrome Virus Nucleocapsid Protein
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Yun Sang-Im
Choi Yu-Jeong
Song Byung-Hak
Kang Shien-Young
Lee Young-Min
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Abstract
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Porcine reproductive and respiratory syndrome virus (PRRSV) is a small enveloped, positive-stranded RNA virus belonging to the family Arteriviridae. It causes the porcine reproductive and respiratory syndrome in swine. The virus has 7 structural proteins: Of the seven, the N protein is the nucleocapsid that comprises a core of the virus particle. We have expressed the N protein of PRRSV PL97-1/LP1 strain using a heterologous gene expression vector derived from Sindbis virus, called pSinrep5. Immunofluorescence analysis showed that the N proteins were mainly found in the cytoplasm as well as in the nucleus of BHK-21 cells transfected with pSinrep5-N-derived RNA. Moreover, expression of the N protein did not change the incompetence of RNA replication of Mutant/nt14900 that lacks a 3’ cis-acting replication element and the efficiency of RNA replication of Mutant/nt14800 that has a low level of RNA replication. Overall, our findings are consistent with previous results and help to understand a role of the N protein in PRRSV biology.
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KEYWORD
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Porcine Reproductive and Respiratory Syndrome Virus, RNA virus, Nucleocapsid, Immunofluorescence
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